ANALYSIS AND CHEMICAL PROFILING OF HONEY USING 1H-NMR SPECTROSCOPY, FTIR SPECTROSCOPY AND TLC USING VARIOUS CHROMOGENIC REAGENTS FOR DERIVATIZATION

Objective: Honey is a natural sweet substance known for various health benefits and used in many traditional medicines dietary supplements. It contains bioactive constituents like sugars, amino acids, polyphenols, flavonoids minerals. Quality control of honey an essential part ensuring its therapeutic usage. In the present study, was analyzed by using spectroscopic approaches physicochemical methods. Methods: The samples were Thin-layer Chromatography (TLC) derivatization, ATR-FTIR, 1H-NMR fingerprint total phenolic content (TPC) flavonoid contents (TFC) measured Uv-Vis spectrophotometry analysis carried out parameters honey. Results: All as per desired quality. UV-Vis successfully determination phenolics TLC showed presence flavonoids, phytosterols, phenolics, sugars carbohydrates Thin-Layer chromatography good resolution components on plates. FTIR functional groups characteristic which further supported 1H NMR chemical profiling. Conclusion: work, application techniques tests found to be useful